Plasmid
Part:BBa_I713018:Design
Designed by: Zhenyu Shi Group: iGEM07_Tsinghua (2007-10-25)
pLZCI3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1723
Illegal PstI site found at 1717 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 673
Illegal PstI site found at 1717
Illegal NotI site found at 1729 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 688
Illegal XhoI site found at 684 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1723
Illegal PstI site found at 1717 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1723
Illegal PstI site found at 1717
Illegal NgoMIV site found at 132 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1463
Illegal BsaI.rc site found at 3105
Illegal SapI site found at 2022
Design Notes
By ligate BBa_I713018 with annealed primers.
Source
BBa_I713018