Plasmid

Part:BBa_I713018:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-25)


pLZCI3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1723
    Illegal PstI site found at 1717
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 673
    Illegal PstI site found at 1717
    Illegal NotI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
    Illegal XhoI site found at 684
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1723
    Illegal PstI site found at 1717
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1723
    Illegal PstI site found at 1717
    Illegal NgoMIV site found at 132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1463
    Illegal BsaI.rc site found at 3105
    Illegal SapI site found at 2022


Design Notes

By ligate BBa_I713018 with annealed primers.


Source

BBa_I713018

References